Escola Paulista de Medicina
Programa de Pós-Graduação em Urologia

Lipidomics analysis of follicular fluid by ESI-MS reveals potential biomarkers for ovarian endometriosis

jarg 2015

Authors:

Fernanda Bertuccez Cordeiro, Thais Regiani Cataldi, Kayla Jane Perkel, Lívia do Vale Teixeira da Costa, Raquel Cellin Rochetti, Juliana Stevanato, Marcos Nogueira Eberlin, Daniel Suslik Zylbersztejn, Agnaldo Pereira Cedenho, Edson Guimarães Lo Turco

Abstract:

Purpose

The aim of the present study was to analyze the lipid profile of follicular fluid from patients with endometriosis and endometrioma who underwent in vitro fertilization treatment (IVF).

Methods

The control group (n = 10) was composed of women with tubal factor or minimal male factor infertility who had positive pregnancy outcomes after IVF. The endometriosis group consisted of women with endometriosis diagnosed by videolaparoscopy (n = 10), and from the same patients, the endometriomas fluids were collected, which composed the endometrioma group (n = 10). From the follicular fluid and endometriomas, lipids were extracted by the Bligh and Dyer method, and the samples were analyzed by tandem mass spectrometry.

Results

We observed phosphatidylglycerol phosphate, phosphatidylcholine, phosphatidylserine, and phosphatidylnositol bisphosphate in the control group. In the endometriosis group, sphingolipids and phosphatidylcholines were more abundant, while in the endometrioma group, sphingolipids and phosphatidylcholines with different m/z from the endometriosis group were found in high abundance.

Conclusion

This analysis demonstrated that there is a differential representation of these lipids according to their respective groups. In addition, the lipids found are involved in important mechanisms related to endometriosis progress in the ovary. Thus, the metabolomic approach for the study of lipids may be helpful in potential biomarker discovery.

DOI:10.1007/s10815-015-0592-1

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